Catalog No: OKCA01607
Size:96 Wells
Price: $857.00
SKU
OKCA01607
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMonkey
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit PrincipleAviva Systems Biology GSTP1 ELISA Kit (Monkey) (OKCA01607) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GSTP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GSTP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GSTP1 captured in the well.
ELISA Kit Range6.25 - 400 ng/mL
ELISA Kit Component
ComponentAmount
GSTP1 Microplate96 Wells (12 x 8 Well strips)
GSTP1 Lyophilized Standard2 x 400 ng
100X Biotinylated GSTP1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity1.56 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolGSTP1
Gene Full NameGlutathione S-transferase P
Alias SymbolsDFN7, FAEES3, GST3, GSTP, PI, deafness, X-linked 7|fatty acid ethyl ester synthase III|glutathione transferase
NCBI Gene Id721704
Protein NameGlutathione S-transferase P
Description of TargetConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
Uniprot IDQ28514
Protein Accession #NP_001036141.1
Nucleotide Accession #NM_001042676.1
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