GSTP1 ELISA Kit (Human) (OKCD00266)

Catalog No: OKCD00266 (Formerly GWB-KBBTW7)

Size:96WELLS

Human GSTP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	84-92%	80-93%	96-103%	95-105%
  EDTA plasma(n=5)	80-103%	90-97%	85-101%	85-102%
  heparin plasma(n=5)	98-105%	94-102%	92-101%	81-93%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Pi (GSTp). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Pi (GSTp). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Pi (GSTp), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione S Transferase Pi (GSTp) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	84-93	89
  EDTA plasma(n=5)	89-96	92
  heparin plasma(n=5)	83-95	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Pi (GSTp) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Pi (GSTp) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GSTP1 Microplate: 96 Wells (12 x 8 Well Strips)
  • GSTP1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated GSTP1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.062 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Pi (GSTp). No significant cross-reactivity or interference between Glutathione S Transferase Pi (GSTp) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GSTP1
• Gene Full Name: glutathione S-transferase pi 1
• Alias Symbols: deafness, X-linked 7;DFN7;epididymis secretory protein Li 22;FAEES3;fatty acid ethyl ester synthase III;glutathione S-transferase P;GST class-pi;GST3;GSTP;GSTP1-1;HEL-S-22;PI.
• NCBI Gene Id: 2950
• Protein Name: Glutathione S-transferase P
• Description of Target: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
• Uniprot ID: P09211
• Protein Accession #: NP_000843
• Nucleotide Accession #: NM_000852.3
• Protein Size (# AA): 210