GSTP1 ELISA Kit (Human) : 96 Wells (OKEH00756)

Catalog No: OKEH00756

Size:96T

Human GSTP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology GSTP1 ELISA Kit (Human) (OKEH00756) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human GSTP1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GSTP1 and incubated. The GSTP1 found in the sample or standards competes with the biotinylated GSTP1 for limited binding sites on the immobilized anti-human GSTP1 antibody. Excess unbound biotinylated GSTP1 and sample or standard GSTP1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GSTP1 captured in the well and inversely proportional to the amount of GSTP1 which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.6% (n = 20)
  Mean Inter-assay CV%: < 8.9% (n = 20)
• ELISA Kit Component:
  • Anti-GSTP1 Microplate: 96 Wells (12 x 8 Well strips)
  • GSTP1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X GSTP1-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.048 ng/mL
• Specificity: Natural and recombinant Human Glutathione S-transferase P
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: GSTP1
• Gene Full Name: glutathione S-transferase pi 1
• Alias Symbols: deafness, X-linked 7;DFN7;epididymis secretory protein Li 22;FAEES3;fatty acid ethyl ester synthase III;glutathione S-transferase P;GST class-pi;GST3;GSTP;GSTP1-1;HEL-S-22;PI.
• NCBI Gene Id: 2950
• Protein Name: P09211
• Description of Target: Glutathione S-transferase P
• Uniprot ID: https://www.uniprot.org/uniprot/P09211
• Protein Accession #: NP_000843.1
• Nucleotide Accession #: NM_000852.3
• Protein Size (# AA): 210
• Molecular Weight: 23 kDa