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Catalog No: OKCD02580
Size:96WELLS
Price: $625.00
SKU
OKCD02580
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Datasheets/ManualsPrintable datasheet for GSTO1 ELISA Kit (Mouse) (OKCD02580)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)98-105%86-93%94-104%96-103%
EDTA Plasma (n=5)85-97%89-104%79-102%78-92%
Heparin Plasma (n=5)94-105%99-105%78-90%86-95%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Omega 1 (GSTo1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Omega 1 (GSTo1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Omega 1 (GSTo1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione S Transferase Omega 1 (GSTo1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)91-10496
EDTA Plasma (n=5)94-10197
Heparin Plasma(n=5)85-9992
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Omega 1 (GSTo1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Omega 1 (GSTo1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GSTO1 Microplate96 Wells (12 x 8 Well strips)
GSTO1 Lyophilized Standard2
100X Biotinylated GSTO1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity0.063 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Omega 1 (GSTo1). No significant cross-reactivity or interference between Glutathione S Transferase Omega 1 (GSTo1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolGsto1
Gene Full Nameglutathione S-transferase omega 1
Alias SymbolsAA407097;AI194287;AU018802;glutathione S-transferase omega 1-1;glutathione S-transferase omega-1;Glutathione transferase omega 1 (GSTO 1-1) (p28);glutathione-dependent dehydroascorbate reductase;glutathione-S-transferase like;GST;Gsto-1;Gstx;MMA(V) reductase;monomethylarsonic acid reductase;p2;p28;S-(Phenacyl)glutathione reductase;Spg-r.
NCBI Gene Id14873
Protein NameGlutathione S-transferase omega-1
Description of TargetExhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.
Uniprot IDO09131
Protein Accession #NP_034492.1
Nucleotide Accession #NM_010362.3
Protein Size (# AA)240
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