GSTM1 ELISA Kit (Human) (OKCD01111)

Catalog No: OKCD01111 (Formerly GWB-KBBIW8)

Size:96WELLS

Human GSTM1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	78-101%	87-95%	81-97%	85-104%
  EDTA plasma(n=5)	79-95%	90-101%	90-105%	81-104%
  heparin plasma(n=5)	83-104%	97-105%	83-104%	81-96%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Mu 1 (GSTM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Mu 1 (GSTM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Mu 1 (GSTM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione S Transferase Mu 1 (GSTM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	79-91	85
  EDTA plasma(n=5)	89-101	98
  heparin plasma(n=5)	79-94	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Mu 1 (GSTM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Mu 1 (GSTM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GSTM1 Microplate: 96 Wells (12 x 8 Well Strips)
  • GSTM1 Lyophilized Standard: 2 x 200 ng
  • 100X Biotinylated GSTM1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 1.16 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Mu 1 (GSTm1). No significant cross-reactivity or interference between Glutathione S Transferase Mu 1 (GSTm1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GSTM1
• Gene Full Name: glutathione S-transferase mu 1
• Alias Symbols: glutathione S-alkyltransferase;glutathione S-aralkyltransferase;glutathione S-aryltransferase;glutathione S-transferase M1;glutathione S-transferase Mu 1;GST class-mu 1;GST HB subunit 4;GST1;GSTM1-1;GSTM1a-1a;GSTM1b-1b;GTH4;GTM1;H-B;HB subunit 4;MU;MU-1;S-(hydroxyalkyl)glutathione lyase.
• NCBI Gene Id: 2944
• Protein Name: Glutathione S-transferase Mu 1
• Description of Target: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
• Uniprot ID: P09488
• Protein Accession #: NP_058710
• Nucleotide Accession #: NM_017014.1
• Protein Size (# AA): 218