Catalog No: OKCD01618 (Formerly GWB-KBBMX3)
Size:96WELLS
Price: $650.00
SKU
OKCD01618
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Datasheets/ManualsPrintable datasheet for GSTa5 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)86-103%84-98%79-101%99-105%
EDTA Plasma (n=5)87-105%84-91%90-97%87-101%
Heparin Plasma (n=5)97-105%94-102%79-95%89-97%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Alpha 5 (GSTa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Alpha 5 (GSTa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Alpha 5 (GSTa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione S Transferase Alpha 5 (GSTa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)94-10297
EDTA Plasma (n=5)91-9996
Heparin Plasma (n=5)84-9388
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Alpha 5 (GSTa5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Alpha 5 (GSTa5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GSTA5 Microplate96 Wells (12 x 8 Well Strips)
GSTA5 Lyophilized Standard2 x 40 ng
100X Biotinylated GSTA5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 0.235 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Alpha 5 (GSTa5). No significant cross-reactivity or interference between Glutathione S Transferase Alpha 5 (GSTa5) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGSTA5
Gene Full NameGlutathione S-transferase alpha 5
Alias SymbolsGlutathione S-transferase A5, Glutathione S-transferase A5-5, GST class-alpha member 5
NCBI Gene Id221357
Protein NameGlutathione S-transferase A5
Description of TargetThe glutathione S-transferases (GST; EC 2.5.1.18) catalyze the conjugation of reduced glutathiones and a variety of electrophiles, including many known carcinogens and mutagens. The cytosolic GSTs belong to a large superfamily, with members located on different chromosomes. For additional information on GSTs, see GSTA1 (MIM 138359).[supplied by OMIM, Sep 2008]
Uniprot IDQ7RTV2
Protein Accession #NP_714543.1
Nucleotide Accession #NM_153699.1
Protein Size (# AA)222
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