Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH02761
Size:96T
Price: $525.00
SKU
OKEH02761
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

Gsta4 ELISA Kit (Rat) : 96 Wells (OKEH02761)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Gsta4 ELISA Kit (Rat) (OKEH02761) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Gsta4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gsta4 and incubated. The Gsta4 found in the sample or standards competes with the biotinylated Gsta4 for limited binding sites on the immobilized anti-rat Gsta4 antibody. Excess unbound biotinylated Gsta4 and sample or standard Gsta4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gsta4 captured in the well and inversely proportional to the amount of Gsta4 which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.3% (n = 20)
Mean Inter-assay CV%: < 10.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Gsta4 Microplate96 Wells (12 x 8 Well strips)
Gsta4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Gsta4-Biotin Complex1 x 60 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.442 ng/mL
SpecificityNatural and recombinant Rat Glutathione S-transferase alpha-4
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolGsta4
Gene Full Nameglutathione S-transferase alpha 4
Alias Symbolsglutathione S-transferase A4;glutathione S-transferase alpha-4;glutathione S-transferase Yk;glutathione transferase;GST 8-8;GST A4-4;GST K;GST Yk.
NCBI Gene Id300850
Protein NameP14942
Description of TargetGlutathione S-transferase alpha-4
Uniprot IDhttps://www.uniprot.org/uniprot/P14942
Protein Accession #NP_034487.2
Nucleotide Accession #NM_010357.3
Protein Size (# AA)222
Molecular Weight26 kDa
Write Your Own Review
You're reviewing:Gsta4 ELISA Kit (Rat) : 96 Wells (OKEH02761)
Your Rating
We found other products you might like!