GSTA3 ELISA Kit (Rat) (OKCD06648)

Catalog No: OKCD06648

Size:96WELLS

GSTA3 ELISA Kit (Rat) (OKCD06648) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Alpha 3 (GSTa3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Alpha 3 (GSTa3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Alpha 3 (GSTa3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione S Transferase Alpha 3 (GSTa3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Alpha 3 (GSTa3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Alpha 3 (GSTa3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GSTA3 Microplate: 96 Wells (12 x 8 Well strips)
  • GSTA3 Lyophilized Standard: 2
  • 100X GSTA3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.133ng/mL

Target Info

• Gene Symbol: Gsta1|LOC108348061
• Gene Full Name: glutathione S-transferase alpha 1|glutathione S-transferase alpha-3
• Alias Symbols: glutathione S-transferase A1;glutathione S-transferase A3 subunit;glutathione S-transferase A5;glutathione S-transferase alpha-3;Glutathione S-transferase Yc-1;glutathione S-transferase Yc1 subunit;Glutathione-S-transferase alpha type (Ya);GST 2-2;GST A3-3;GST AA;GST Yc1;Gsta3;Gsta5;Yc1.
• NCBI Gene Id: 108348061|24421
• Protein Name: Glutathione S-transferase alpha-3
• Description of Target: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.
• Uniprot ID: P04904
• Protein Accession #: NP_113697.1
• Nucleotide Accession #: NM_031509.2