GSTA2 ELISA Kit (Human) (OKCD00853)

Catalog No: OKCD00853 (Formerly GWB-KBBMM8)

Size:96WELLS

Human GSTA2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	80-91%	95-103%	82-105%	91-98%
  EDTA plasma(n=5)	94-102%	93-101%	93-103%	78-89%
  heparin plasma(n=5)	78-96%	96-103%	85-105%	81-88%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Alpha 2 (GSTa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Alpha 2 (GSTa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Alpha 2 (GSTa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione S Transferase Alpha 2 (GSTa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	90-97	93
  EDTA plasma(n=5)	78-102	91
  heparin plasma(n=5)	78-96	82

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Alpha 2 (GSTa2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Alpha 2 (GSTa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GSTA2 Microplate: 96 Wells (12 x 8 Well Strips)
  • GSTA2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated GSTA2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.125 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Alpha 2 (GSTa2). No significant cross-reactivity or interference between Glutathione S Transferase Alpha 2 (GSTa2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GSTA2
• Gene Full Name: glutathione S-transferase alpha 2
• Alias Symbols: glutathione S-alkyltransferase A2;glutathione S-aralkyltransferase A2;glutathione S-aryltransferase A2;glutathione S-transferase A2;GST class-alpha member 2;GST HA subunit 2;GST2;GSTA2-2;GST-gamma;GTA2;GTH2;liver GST2;S-(hydroxyalkyl)glutathione lyase A2;testis tissue sperm-binding protein Li 59n.
• NCBI Gene Id: 2939
• Protein Name: Glutathione S-transferase A2
• Description of Target: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
• Uniprot ID: P09210
• Protein Accession #: NP_000837
• Nucleotide Accession #: NM_000846.4
• Protein Size (# AA): 222