GSN Chemi-Luminescent ELISA Kit (Human) (OKCD03544)

Catalog No: OKCD03544

Size:96WELLS

Human GSN ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-104%	78-89%	78-99%	80-91%
  EDTA Plasma (n=5)	84-99%	98-105%	98-105%	97-104%
  Heparin Plasma (n=5)	98-105%	84-98%	84-103%	82-98%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Gelsolin (GSN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Gelsolin (GSN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Gelsolin (GSN) level in the sample or standard.
• ELISA Kit Range: 13.7-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-96	85
  EDTA Plasma (n=5)	84-92	89
  Heparin Plasma (n=5)	84-102	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gelsolin (GSN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gelsolin (GSN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GSN Microplate: 96 Wells (12 x 8 Well strips)
  • GSN Lyophilized Standard: 2
  • 100X GSN Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 1.37 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GSN
• Gene Full Name: gelsolin
• Alias Symbols: actin-depolymerizing factor;ADF;AGEL;brevin;epididymis secretory sperm binding protein;gelsolin.
• NCBI Gene Id: 2934
• Protein Name: Gelsolin
• Description of Target: Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
• Uniprot ID: P06396
• Protein Accession #: NP_000168
• Nucleotide Accession #: NM_000177