GSK3A Antibody (OAAF01317)

Catalog No: OAAF01317

Size:100 ug

Western blot analysis of extracts from K562/HepG2/RAW264.7 cells, using GSK3 alpha Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 alpha Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human GSK3A.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: GSK3α(Ab-21) Antibody detects endogenous levels of GSK3α protein around Serine 21.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000; IP: Various Dilution;
• Reference: Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
  Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760.
  Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279.
  Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.
  Cross

Target Info

• Gene Symbol: GSK3A
• Gene Full Name: glycogen synthase kinase 3 alpha
• Alias Symbols: glycogen synthase kinase-3 alpha;GSK-3 alpha;serine/threonine-protein kinase GSK3A.
• NCBI Gene Id: 2931
• Protein Name: Glycogen synthase kinase-3 alpha
• Description of Target: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.
• Uniprot ID: P49840
• Protein Accession #: NP_063937.2
• Molecular Weight: 50 kDa