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Catalog No: OKEH06424
Size:96T
Price: $525.00
SKU
OKEH06424
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology GSDMC ELISA Kit (Human) (OKEH06424) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-GSDMC antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GSDMC and incubated. The GSDMC found in the sample or standards competes with the biotinylated GSDMC for limited binding sites on the immobilized anti-GSDMC antibody. Excess unbound biotinylated GSDMC and sample or standard GSDMC is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GSDMC captured in the well and inversely proportional to the amount of GSDMC which was contained in the sample or standard.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 96%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 6.4% (n = 20)
Mean Inter-Assay Precision - <= 11.1% (n = 20)
ELISA Kit Component
ComponentAmount
H3F3C Microplate96 Wells (12 x 8 Well strips)
H3F3C Lyophilized Standard2
100X Biotinylated H3F3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.319 ng/mL
SpecificityNatural and recombinant Human Gasdermin-C
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolGSDMC
Gene Full Namegasdermin C
Alias Symbolsgasdermin-C;melanoma-derived leucine zipper, extra-nuclear factor;melanoma-derived leucine zipper-containing extranuclear factor;MLZE.
NCBI Gene Id56169
Protein NameQ9BYG8
Description of TargetGasdermin-C
Uniprot IDhttps://www.uniprot.org/uniprot/Q9BYG8
Protein Accession #NP_062379
Nucleotide Accession #NM_019506
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