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Catalog No: OKEH04772 (Formerly GWB-HMCMQH)
Size:96T
Price: $525.00
SKU
OKEH04772
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology GSAP ELISA Kit (Human) (OKEH04772) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GSAP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GSAP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GSAP captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 112%
ELISA Kit ReproducibilityMean Intra-Assay CV: < 6.5%
Mean Inter-Assay CV: < 11.6%
ELISA Kit Component
ComponentAmount
GSAP Microplate96 Wells (12 x 8 Well Strips)
GSAP Standard2
Sample Diluent1 x 20 mL
GSAP Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.78 ng/mL
SpecificityNatural and recombinant Human Gamma-secretase-activating protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGSAP
Gene Full Namegamma-secretase activating protein
Alias Symbolsgamma-secretase-activating protein;pigeon homolog;PION;protein pigeon homolog.
NCBI Gene Id54103
Protein NameA4D1B5
Description of TargetGamma-secretase-activating protein
Uniprot IDhttps://www.uniprot.org/uniprot/A4D1B5
Protein Accession #NP_059135.2
Nucleotide Accession #NM_017439.3
Protein Size (# AA)854
Molecular Weight98 kDa
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