GRP94 Antibody : Biotin (OASE00006-Biotin)

Catalog No: OASE00006-Biotin

Size:200ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea pig, Hamster, Horse, Monkey, Pig, Rabbit, Sheep, Xenopus
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 9G10
• Isotype: IgG2a
• Host: Rat
• Conjugation: Biotin
• Application: WB, IP, FC
• Additional Information: Background Info: Detects a 98kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. Does not detect human Hsp90, Grp74, or GrpE from E.coli.
  Scientific Background: Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2) Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homology with its cytosolic equivalent, Hsp90 (3). Both Hsp90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity (5).
  Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
  Certificate of Analysis: 0.5 ug/mL was sufficient for detection of Grp94 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Purified Grp94 isolated from chicken oviducts
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 0.5ug/ml was sufficient for detection of Grp94 in 20ug of HeLa lysate.
• Storage Buffer: PBS pH7.2, 50% glycerol
• Reference: 1. Rudolph R.W., and Bedows E. (1997) J Biol Chem 272: 3125-3128.
  2. Srivastava P.K., et al. (1994) Immunogenetics. 39(2):93-
  98.
  3. Mazzarella R.A., and Green M. (1987) J Biol Chem 262:
  8875-8883.
  4. Kang, H.S. and Welch W.J. (1991) J Biol Chem 266(9):
  5643-5649.
  5. Soldano K.L., et al. (2003) J Biol Chem 278(48): 48330-
  48338.
  6. Chu F., et al. (2006) Protein Sci 15(6): 1260-1269.
  7. Peter F., et al., (1992) J Biol Chem 267: 10631-10637.
  8. Allen S. et al. (2000) Blood 96(2): 560-568.
  9. Sato K et al. (2001) Blood 98(6): 1852-1857.
  10. Yun S.-W. et al (2000) Brain Research Bulletin.52(5):
  371-378.
  11. Choukhi A., et al. (1998) J. Virol. 72: 3851-3858.
  12. Hoshino T., et al. (1998) Blood 91(11): 4379-4386.
  13. Riera M. et al. (1999) Mol. Cell Biochem. 191: 97-104.
  14. Gusarova V., et al. (2001) J. Biol. Chem. 276(27): 24891-
  24900.

Target Info

• Gene Symbol: HSP90B1
• Gene Full Name: heat shock protein 90kDa beta (Grp94), member 1
• Alias Symbols: ECGP, GP96, TRA1, GRP94, HEL35, HEL-S-125m
• NCBI Gene Id: 7184
• Uniprot ID: P14625
• Protein Accession #: NP_003290.1