Grp78 Recombinant Protein (Hamster) (OPED00050)

Catalog No: OPED00050

Size:50 ug

SDS-PAGE analysis: Lane 1: MWM, Lane 2: 0.5ug, Lane 3: 1ug, Lane 4: 2ug, Lane 5: 5ug of Recombinant Grp78 Hamster Protein

Product Info

• Predicted Species Reactivity: Hamster|Mesocricetus auratus
• Product Format: Liquid. In 50mM Tris, pH 7.5, containing 150mM NaCl, 10% glycerol and 1mM DTT
• Host: E. Coli
• Application: WB
• Additional Information: Grp78, a 78 kDa glucose-regulated protein also known as BiP or immunoglobulin heavy chain binding protein, is a stress-response protein induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding and assembly of many membrane bound and secreted proteins. Grp78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. Grp78 protein levels provide a reliable biomarker of hypoglycemia as well as serve a neuroprotective function in neurons exposed to glutamate and oxidative stress. Reduced Grp78 levels occur in the brains of Alzheimer’s Disease patients, and decreased Grp78 expression appears to be associated with missense mutations in the human presenilin-1 (PS1) gene. The induction of the Grp78 protein is associated with the development of drug-resistance to anti-tumor drugs.
• Reconstitution and Storage: Store at -80C
• Purification: Purified by multi-step chromatography.
• Purity: >80% (SDS-PAGE; Western blot)
• Application Info: Western blot control.
• Reference: Selection and identification of ligand peptides targeting a model of castrate-resistant osteogenic prostate cancer and their receptors: J. Mandelin, et al.; PNAS 112, 3776 (2015), Application(s): Western Blotting, Abstract; Full Text
  Tumor imaging and targeting potential of an Hsp70-derived 14-mer peptide: M. Gehrmann, et al.; PLoS One 9, e105344 (2014), Abstract; Full Text
  Differential protein expression during aging in ventricular myocardiumof Fischer 344 x Brown Norway hybrid rats: F. Witzmann, et al. ; Exp. Gerontol. 43, 909 (2008), Abstract;
  Fingerprinting the circulating repertoire of antibodies from cancer patients: W. Arap, et al. ; Nat. Biotechnol. 21, 57 (2003), Application(s): WB , Abstract;
  Coupling endoplasmic reticulum stress to the cell death program: role of the ER chaperone GRP78: D.E. Bredesen, et al. ; FEBS Lett. 514, 122 (2002), Application(s): WB , Abstract;
  The dynamic role of GRP78/BiP in the coordination of mRNA translation with protein processing: C.O. Brostrom, et al. ; J. Biol. Chem. 274, 486 (1999), Application(s): WB , Abstract;

Target Info

• Gene Symbol: GRP78
• Alias Symbols: 78 kDa glucose-regulated protein;Binding-immunoglobulin protein;Heat shock protein 70 family protein 5;Heat shock protein family A member 5;Immunoglobulin heavy chain-binding protein.
• Protein Name: Endoplasmic reticulum chaperone BiP
• Description of Target: Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (By similarity). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating.
• Uniprot ID: P07823
• Protein Accession #: NP_001158884.1
• Nucleotide Accession #: NM_001165412.1
• Protein Size (# AA): Recombinant
• Molecular Weight: 78 kDa