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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OASE00141-HRP |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Cryphonectria parasitica, Hamster, Monkey, Rabbit, Xenopus |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Monoclonal |
Clone | 1H11-1H7 |
Isotype | IgG2b |
Host | Mouse |
Conjugation | HRP: Horseradish Peroxidase |
Application | WB |
Additional Information | Background Info: Detects a protein with a mass of ~78kDa corresponding to GRP78 |
:: | Scientific Background: GRP78 is a ubiquitously expressed, 78-kDa glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Lou et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7). |
:: | Certificate of Analysis: 0.5 ug/ml was sufficient for detection of Grp78 in 10ug of rat tissue lysate by ECL immunoblot analysis. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | His-tagged human GRP78 |
Purification | Protein G Purified |
Concentration | 0.6-0.7 mg/ml |
Dilution | 1:2000 (WB) |
Storage Buffer | PBS pH7.4, 50% glycerol |
Reference | 1. Cho, S. et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang, Y. et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo, S. et al (2006) 26 (15): 5688-97. 4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi, R. et al. (1999) Anticancer Res. 19:4333- 4336. 6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949. 7. Dong, D. et al. (2005) Cancer Res 65(13): 5785-91. |
Gene Symbol | Hspa5 |
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Gene Full Name | heat shock protein 5 |
Alias Symbols | B, mB, Bip, Grp, Sez, 78kD, Sez7, mBiP, Grp78, Hsce7, SEZ-7, baffl, Hsce70, D2Wsu17, baffled, AL022860, AU019543, D2Wsu141, D2Wsu17e, D2Wsu141e |
NCBI Gene Id | 14828 |
Uniprot ID | P20029 |
Protein Accession # | NP_001156906.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GRP78 Antibody : HRP (OASE00141-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Cryphonectria parasitica, Hamster, Monkey, Rabbit, Xenopus".
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How long will it take to receive "GRP78 Antibody : HRP (OASE00141-HRP)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "GRP78 Antibody : HRP (OASE00141-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GRP78 Antibody : HRP (OASE00141-HRP)"?
This target may also be called "B, mB, Bip, Grp, Sez, 78kD, Sez7, mBiP, Grp78, Hsce7, SEZ-7, baffl, Hsce70, D2Wsu17, baffled, AL022860, AU019543, D2Wsu141, D2Wsu17e, D2Wsu141e" in publications.
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What is the shipping cost for "GRP78 Antibody : HRP (OASE00141-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GRP78 Antibody : HRP (OASE00141-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GRP78 Antibody : HRP (OASE00141-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GRP78 Antibody : HRP (OASE00141-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HSPA5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HSPA5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HSPA5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HSPA5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HSPA5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HSPA5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.