GRP ELISA Kit (Mouse) (OKEH06991)

Catalog No: OKEH06991

Size:96T

Mouse GRP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Grp ELISA Kit (Mouse) (OKEH06991) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Grp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Grp and incubated. The Grp found in the sample or standards competes with the biotinylated Grp for limited binding sites on the immobilized anti-Grp antibody. Excess unbound biotinylated Grp and sample or standard Grp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Grp captured in the well and inversely proportional to the amount of Grp which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 88%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <3.9%
  Inter-Assay CV <6.7%
• ELISA Kit Component:
  • Grp Microplate: 96 Wells (12 x 8 Well strips)
  • Grp Lyophilized Standard: 2 x 20 ng
  • 100X Grp HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Mouse Gastrin-releasing peptide
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Grp
• Gene Full Name: gastrin releasing peptide
• Alias Symbols: B;BLP;gastrin-releasing peptide.
• NCBI Gene Id: 225642
• Protein Name: Q8R1I2
• Description of Target: Gastrin-releasing peptide
• Uniprot ID: https://www.uniprot.org/uniprot/Q8R1I2
• Protein Accession #: NP_778177
• Nucleotide Accession #: NM_175012