Growth Hormone ELISA Kit (Human) (OKBB00284)
|Predicted Species Reactivity||Human|
|Kit Detection Method||Colorimetric, OD450 nm|
|Kit Duration||~ 3 Hours|
|Kit Principle||Aviva Systems Biology GH1 ELISA Kit (Human) (OKBB00284) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for GH1 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (E.coli, F27-F217) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GH1 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human GH1 captured in well.|
|Kit Range||15.6 pg/mL-1,000 pg/mL|
|Kit Component||1. Lyophilized recombinant human E-Cadherin standard: 10ng/tube×2.2. One 96-well plate precoated with anti- human E-Cadherin antibody.3. Sample diluent buffer: 30 ml4. Biotinylated anti- human E-Cadherin antibody : 130ul, dilution 1:100.5. Antibody diluent buffer: 12ml.6. Avidin-Biotin-Peroxidase Complex 7. ABC diluent buffer: 12ml.(ABC): 130ul, dilution 1:100.8. TMB color developing agent: 10ml.,9. TMB stop solution: 10ml.|
|Additional Information||Range: 15.6pg/ml-1000pg/ml|
|::||Principle: Aviva’s human Growth Hormone ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Growth Hormone specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Growth Hormone amount of sample captured in plate.|
|::||Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
Background: Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.
|Reconstitution and Storage||Store as indicated in product manual.|
|Sample Type||cell culture supernates, serum and plasma (heparin, EDTA)|
|Sensitivity||< 2 pg/mL|
|Predicted Homology Based on Immunogen Sequence||No detectable cross-reactivity with any other cytokine|
|Datasheets/Manuals||Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.|
|Target Reference||1. Baum, H. B. A.; Katznelson, L.; Sherman, J. C.; Biller, B. M. K.; Hayden, D. L.; Schoenfeld, D. A.; Cannistraro, K. E.;
Klibanski, A. : Effects of physiological growth hormone (GH) therapy on cognition and quality of life in patients with
adult-onset GH deficiency. J. Clin. Endocr. Metab. 83: 3184-3189, 1998
2. Niall, H. D.; Hogan, M. L.; Sauer, R.; Rosenblum, I. Y.; Greenwood, F. C. :
Sequence of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene
reduplication. Proc. Nat. Acad. Sci. 68: 866-869, 1971.
3. Owerbach, D.; Rutter, W. J.; Martial, J. A.; Baxter, J. D.; Shows, T. B. : Genes for growth hormone, chorionic
somatomammotropin and growth hormone-like genes on chromosome 17 in humans. Science 209: 289-292, 1980.
|Specificity||There is no detectable cross-reactivity with other relevant proteins.|
|Official Gene Full Name||growth hormone 1|
|Alias Symbols||Somatotropin, Growth Hormone 1, GH1|
|NCBI Gene Id||2688|
|Description of Target||Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life.|
|Protein Accession #||NP_000506.2|
|Nucleotide Accession #||NM_000515.4|
|Tissue Tool||Find tissues and cell lines supported by DNA array analysis to express GH1.|
|RNA Seq||Find tissues and cell lines supported by RNA-seq analysis to express GH1.|
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
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