Catalog No: OPPA01064 (Formerly GWB-9D97DE)
Size:10UG
Price: $75.00
SKU
OPPA01064
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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GroEL E.Coli Protein (OPPA01064)
GroEL protein is the major heat shock protein of E.coli and belongs to the chaperonin (HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition. GroEL gene was amplified by PCR from E.coli and cloned into an expression vector.
Predicted Species Reactivity | Escherichia coli |
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Product Format | Liquid 25mM Tris-HCl buffer (pH 7.5), 100mM NaCl, 1mM DTT and 10% Glycerol. |
Host | E. Coli |
Reconstitution and Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
Purification | GroEL is purified by proprietary chromatographic techniques. |
Concentration | 1 mg/ml |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREIELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGIDKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDGTGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAVAKAGKPLLII AEDVEGEALA TAVVNTIRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTVISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDYDREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALIRVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNAATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM. |
Gene Symbol | GroEL |
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Alias Symbols | ECK4137, groEL, mopA |
NCBI Gene Id | 948665 |
Protein Name | 60 kDa chaperonin |
Description of Target | Recombinant E.Coli GroEL (HSP60) |
Uniprot ID | P0A6F5 |
Protein Accession # | AP_004644.1 |
Protein Size (# AA) | Recombinant |
Molecular Weight | 57 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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