GRO b Protein (OPPA00435)

Catalog No: OPPA00435 (Formerly GWB-66CEC5)

Size:5UG

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: The protein was lyophilized with no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized GRO-beta Mouse in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Biological Activity: The Biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with >5 ng/ml of recombinant mouse MIP-2.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.015 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GRO-b as a Reference Standard.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.015 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GRO-b as a Reference Standard.
  Product Introduction: Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.
  Product Description: GRO-Beta Mouse Recombinant also caled mouse MIP-2 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7849 Dalton. The CXCL2 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized CXCL2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CXCL2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Ala - Val - Val - Ala - Ser.

Target Info

• Gene Symbol: mGRO b
• Alias Symbols: Gr, Mi, MIP, Scy, CINC, GROb, Gro2, Mgsa, Mip2, Scyb, MIP-2, Scyb2, MIP-2a, Mgsa-b, CINC-2a
• NCBI Gene Id: 20310
• Protein Name: C-X-C motif chemokine 2
• Description of Target: Recombinant Mouse GRO-beta/MIP-2 (CXCL2)
• Uniprot ID: P10889
• Protein Accession #: NP_033166.1
• Protein Size (# AA): Recombinant