Grn ELISA Kit (Mouse) (OKBB01529)

Catalog No: OKBB01529

Size:96 wells

Grn ELISA Kit (Mouse) (OKBB01529) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse Progranulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Progranulin has been precoated onto 96-well plates. Standards(NS0, T18-L589) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Progranulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Progranulin amount of sample captured in plate.
• ELISA Kit Range: 0.78 ng/ml - 50 ng/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	2007	7073	23934	1896	6992	22153
  Standard deviation	130.45	339.5	1006.5%2	144.09	349.6	1019.03
  CV(%)	6.5	4.8	4.2	7.6	5	4.6

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Mouse Grn Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Mouse Grn Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Mouse Grn Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	2007	2106	1888	1899	1975	88.78	4.4%
  Sample 2	7073	7342	7311	6975	7175	155.55	2.1%
  Sample 3	23934	24448	24758	24233	24343	301.09	1.2%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
  O.D.	0.005	0.217	0.464	0.812	1.418	1.658	2.113	2.275

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates,cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse Progranulin
• Immunogen: Expression system for standard: NS0; Immunogen sequence: T18-L589
• Description: The Quick version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Progranulin/GRN with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.

Target Info

• Gene Symbol: GRN
• Gene Full Name: Granulins
• Alias Symbols: Granulins;PC cell-derived growth factor;PCDGF;Proepithelin;PEPI;Acrogranin;Progranulin;Granulin-1;Granulin-2;Granulin-3;Granulin-4;Granulin-5;Granulin-6;Granulin-7;Grn;
• NCBI Gene Id: 14824
• Protein Name: Granulins
• Description of Target: Granulin, also known as Proepithelin(PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
• Uniprot ID: P28798
• Protein Accession #: NP_032201.2
• Nucleotide Accession #: NM_008175.4