GRK5 Recombinant Protein (OPCD03686)

Catalog No: OPCD03686

GRK5 Recombinant Protein (OPCD03686) using SDS-PAGE

GRK5 Recombinant Protein (OPCD03686) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Arg304-Thr532
  Subcellular Location: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protei
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 200 ug/mL (prior to lyoph)
• Purity: > 95%
• Source: E.coli
• Tag: N-terminal His Tag

Target Info

• Gene Symbol: GRK5
• Gene Full Name: G protein-coupled receptor kinase 5
• Alias Symbols: FP2025;G protein-coupled receptor kinase 5;g protein-coupled receptor kinase GRK5;GPRK5.
• NCBI Gene Id: 2869
• Protein Name: G protein-coupled receptor kinase 5
• Description of Target: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).
• Uniprot ID: P34947
• Protein Accession #: NP_005299.1
• Nucleotide Accession #: NM_005308.2
• Protein Size (# AA): Arg304~Thr532
• Molecular Weight: 30kDa