GRIN2A ELISA Kit (Human) (OKCD04531)

Catalog No: OKCD04531

Size:96WELLS

GRIN2A ELISA Kit (Human) (OKCD04531) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h, 30min
• ELISA Kit Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A Antibody (Anti-GRIN2A) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A Antibody (Anti-GRIN2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A Antibody (Anti-GRIN2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A Antibody (Anti-GRIN2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GRIN2A Microplate: 96 Wells (12 x 8 Well strips)
  • GRIN2A Lyophilized Standard: 2
  • 100X GRIN2A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 1.33ng/mL

Target Info

• Gene Symbol: GRIN2A
• Gene Full Name: glutamate ionotropic receptor NMDA type subunit 2A
• Alias Symbols: EPND;FESD;GluN2A;Glutamate [NMDA] receptor subunit epsilon-1;glutamate ionotropic receptor NMDA 2A;glutamate receptor ionotropic, NMDA 2A;glutamate receptor, ionotropic, N-methyl D-aspartate 2A;LKS;NMDAR2A;N-methyl D-aspartate receptor subtype 2A;N-methyl-D-aspartate receptor channel, subunit epsilon-1;N-methyl-D-aspartate receptor subunit 2A;NR2A.
• NCBI Gene Id: 2903
• Protein Name: Glutamate receptor ionotropic, NMDA 2A
• Description of Target: Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:8768735, PubMed:26919761, PubMed:26875626, PubMed:28105280). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have higher sensitivity to glutamate and faster kinetics than channels formed by GRIN1 and GRIN2B (PubMed:26919761, PubMed:26875626). Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity).
• Uniprot ID: Q12879
• Protein Accession #: NP_000824.1
• Nucleotide Accession #: NM_000833.4