GRIN1 ELISA Kit (Rat) (OKEH03092)

Catalog No: OKEH03092

Size:96T

Rat GRIN1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology GRIN1 ELISA Kit (Rat) (OKEH03092) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Grin1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Grin1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Grin1 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Component:
  • Anti-Grin1 Microplate: 96 Wells (12 x 8 Well strips)
  • Grin1 Lyophilized Standard: 2
  • 100X Biotinylated Grin1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.162 ng/mL
• Specificity: Natural and recombinant Rat Glutamate receptor ionotropic, NMDA 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Grin1
• Gene Full Name: glutamate ionotropic receptor NMDA type subunit 1
• Alias Symbols: GluN1;glutamate [NMDA] receptor subunit zeta-1;glutamate receptor ionotropic, NMDA 1;glutamate receptor, ionotropic, N-methyl D-aspartate 1;neurotransmitter receptor;NMDA R1 receptor C1 cassette;NMDAR1;NMD-R1;N-methyl-D-aspartate glutamate receptor;N-methyl-D-aspartate receptor subunit NR1;NR1.
• NCBI Gene Id: 24408
• Protein Name: P35439
• Description of Target: Glutamate receptor ionotropic, NMDA 1
• Uniprot ID: https://www.uniprot.org/uniprot/P35439
• Protein Accession #: NP_001257531.1
• Nucleotide Accession #: NM_001270602.1
• Protein Size (# AA): 938
• Molecular Weight: 106 kDa