Aviva Systems Biology office will be closed for Memorial Day - May 27, 2019.
Please go here for more info.

Search Antibody, Protein, and ELISA Kit Solutions

GRIN1 ELISA Kit (Rat) (OKEH03092)

96 Wells
$529.00
In Stock
Request Bulk Order Quote

Gene Symbol:
GRIN1
Official Gene Full Name:
glutamate ionotropic receptor NMDA type subunit 1
NCBI Gene Id:
24408
Alias Symbols:
Nmdar1, NMDAR1, NMD-R1, N-methyl-D-aspartate receptor subunit NR1, NR1
Description of Target:
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
Protein Name:
Glutamate receptor ionotropic- NMDA 1
Swissprot Id:
P35439
Protein Accession #:
NP_001257531.1
Nucleotide Accession #:
NM_001270602.1
Predicted Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
0.156 ng/mL
Kit Range:
0.312 - 20 ng/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology GRIN1 ELISA Kit (Rat) (OKEH03092) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Grin1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Grin1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Grin1 captured in well.
Kit Component:
ComponentAmount
Anti-Grin1 Microplate96 Wells (12 x 8 Well strips)
Grin1 Lyophilized Standard2
100X Biotinylated Grin1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Grin1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Grin1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural rat NMDAR1. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
106 kDa
Protein Size (# AA):
938

Product Reviews

Protocol:
Tips Information:

Tell us what you think about this item!

Write A Review
    Please, wait...