Catalog No: OKEH07855
Size:96T
Price: $725.00
SKU
OKEH07855
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology GRHPR ELISA Kit (Human) (OKEH07855) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GRHPR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GRHPR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GRHPR captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=3.1% (n = 20)
Mean Inter-assay CV%: <=11.1% (n = 20)
ELISA Kit Component
ComponentAmount
GRHPR Microplate96 Wells (12 x 8 Well strips)
GRHPR Lyophilized Standard2
100X Biotinylated GRHPR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Enzyme with hydroxy-pyruvate reductase, glyoxylate reductase and D-glycerate dehydrogenase enzymatic activities. Reduces hydroxypyruvate to D-glycerate, glyoxylate to glycolate oxidizes D-glycerate to hydroxypyruvate.
::Unigene: Hs.155742
SMR: Q9UBQ7
String: 9606.ENSP00000313432
::KEGG: hsa:9380
MIM: 260000
Pfam: PF00389
Reconstitution and StorageStore as indicated in product manual.
Sensitivity40.4pg/mL
SpecificityNatural and recombinant Human Glyoxylate reductase/hydroxypyruvate reductase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGRHPR
Gene Full Nameglyoxylate and hydroxypyruvate reductase
Alias Symbolsepididymis secretory sperm binding protein;GLXR;glycerate-2-dehydrogenase;GLYD;glyoxylate reductase and hydroxypyruvate reductase;glyoxylate reductase/hydroxypyruvate reductase;PH2.
NCBI Gene Id9380
Protein NameQ9UBQ7
Description of TargetGlyoxylate reductase/hydroxypyruvate reductase
Uniprot IDhttps://www.uniprot.org/uniprot/Q9UBQ7
Protein Accession #NP_036335.1
Nucleotide Accession #NM_012203.1
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