Catalog No: OKEH07855
Size:96T
Price: $725.00
SKU
OKEH07855
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology GRHPR ELISA Kit (Human) (OKEH07855) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GRHPR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GRHPR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GRHPR captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=3.1% (n = 20) Mean Inter-assay CV%: <=11.1% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Enzyme with hydroxy-pyruvate reductase, glyoxylate reductase and D-glycerate dehydrogenase enzymatic activities. Reduces hydroxypyruvate to D-glycerate, glyoxylate to glycolate oxidizes D-glycerate to hydroxypyruvate. | ||||||||||||||||||||||
:: | Unigene: Hs.155742 SMR: Q9UBQ7 String: 9606.ENSP00000313432 | ||||||||||||||||||||||
:: | KEGG: hsa:9380 MIM: 260000 Pfam: PF00389 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 40.4pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Glyoxylate reductase/hydroxypyruvate reductase | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | GRHPR |
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Gene Full Name | glyoxylate and hydroxypyruvate reductase |
Alias Symbols | epididymis secretory sperm binding protein;GLXR;glycerate-2-dehydrogenase;GLYD;glyoxylate reductase and hydroxypyruvate reductase;glyoxylate reductase/hydroxypyruvate reductase;PH2. |
NCBI Gene Id | 9380 |
Protein Name | Q9UBQ7 |
Description of Target | Glyoxylate reductase/hydroxypyruvate reductase |
Uniprot ID | https://www.uniprot.org/uniprot/Q9UBQ7 |
Protein Accession # | NP_036335.1 |
Nucleotide Accession # | NM_012203.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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