GREM1 ELISA Kit (Rat) (OKEH06051)

Catalog No: OKEH06051

Size:96T

Rat GREM1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Grem1 ELISA Kit (Rat) (OKEH06051) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Grem1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Grem1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Grem1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 102%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <4.5%, n = 20
  Mean Inter-Assay Precision - <7.8%, n = 20
• ELISA Kit Component:
  • GREM1 Microplate: 96 Wells (12 x 8 Well strips)
  • GREM1 Lyophilized Standard: 2
  • 100X Biotinylated GREM1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.088 ng/mL
• Specificity: Natural and recombinant Rat Gremlin-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Grem1
• Gene Full Name: gremlin 1, DAN family BMP antagonist
• Alias Symbols: Cktsf1b1;cysteine knot superfamily 1, BMP antagonist 1;down-regulated in Mos-transformed cells protein;drm;gremlin 1 homolog, cysteine knot superfamily;gremlin-1.
• NCBI Gene Id: 50566
• Protein Name: O35793
• Description of Target: Gremlin-1
• Uniprot ID: https://www.uniprot.org/uniprot/O35793
• Protein Accession #: NP_062155.1
• Nucleotide Accession #: NM_019282.3
• Protein Size (# AA): 184