Grem1 ELISA Kit (Mouse) (OKEH04782)

Catalog No: OKEH04782

Size:96T

Mouse Grem1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Grem1 ELISA Kit (Mouse) (OKEH04782) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Grem1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Grem1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Grem1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Grem1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Grem1 Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Grem1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.087 ng/mL
• Specificity: Natural and recombinant Mouse Gremlin-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Grem1
• Gene Full Name: gremlin 1, DAN family BMP antagonist
• Alias Symbols: Cktsf1b;Cktsf1b1;cysteine knot superfamily 1, BMP antagonist 1;down-regulated in Mos-transformed cells protein;down-regulated in v-mos-transformed cells;Dr;Drm;gre;Grem;gremlin-1;ld.
• NCBI Gene Id: 23892
• Protein Name: O70326
• Description of Target: Gremlin-1
• Uniprot ID: https://www.uniprot.org/uniprot/O70326
• Protein Accession #: NP_035954.1
• Nucleotide Accession #: NM_011824.4
• Protein Size (# AA): 184
• Molecular Weight: 21 kDa