20% off all ELISAs • Two for the price of one on select antibodies.

Don’t miss out on these great offers! Learn More Here.

Catalog No: OKEH04782
Price: $525.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
Please contact customer service when ordering 10+ kits.

Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Grem1 ELISA Kit (Mouse) (OKEH04782) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Grem1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Grem1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Grem1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
Grem1 Microplate96 Wells (12 x 8 Well Strips)
Grem1 Standard2
Sample Diluent1 x 20 mL
Grem1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.087 ng/mL
SpecificityNatural and recombinant Mouse Gremlin-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGrem1
Gene Full Namegremlin 1, DAN family BMP antagonist
Alias SymbolsCktsf1b;Cktsf1b1;cysteine knot superfamily 1, BMP antagonist 1;down-regulated in Mos-transformed cells protein;down-regulated in v-mos-transformed cells;Dr;Drm;gre;Grem;gremlin-1;ld.
NCBI Gene Id23892
Protein NameO70326
Description of TargetGremlin-1
Uniprot ID
Protein Accession #NP_035954.1
Nucleotide Accession #NM_011824.4
Protein Size (# AA)184
Molecular Weight21 kDa
Write Your Own Review
You're reviewing:Grem1 ELISA Kit (Mouse) (OKEH04782)
Your Rating
We found other products you might like!