Catalog No: OKCD01156 (Formerly GWB-KBBWE3)
Size:96WELLS
Price: $675.00
SKU
OKCD01156
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Datasheets/ManualsPrintable datasheet for GREM1 ELISA Kit (Human) (OKCD01156)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)97-105%78-102%80-96%81-95%
EDTA plasma(n=5)95-103%79-90%96-105%80-97%
heparin plasma(n=5)84-97%85-101%96-104%91-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gremlin 1 (GREM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gremlin 1 (GREM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gremlin 1 (GREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Gremlin 1 (GREM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range6.25-400ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)89-9793
EDTA plasma(n=5)80-8985
heparin plasma(n=5)78-9783
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gremlin 1 (GREM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gremlin 1 (GREM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GREM1 Microplate96 Wells (12 x 8 Well Strips)
GREM1 Lyophilized Standard2 x 40 ng
100X Biotinylated GREM1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.262 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Gremlin 1 (GREM1). No significant cross-reactivity or interference between Gremlin 1 (GREM1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGREM1
Gene Full Namegremlin 1, DAN family BMP antagonist
Alias SymbolsC15DUPq;cell proliferation-inducing gene 2 protein;CKTSF1B1;colorectal adenoma and carcinoma 1;CRAC1;CRCS4;cysteine knot superfamily 1, BMP antagonist 1;DAN domain family member 2;DAND2;down-regulated in Mos-transformed cells protein;DRM;DUP15q;GREMLIN;gremlin 1, cysteine knot superfamily, homolog;gremlin 1-like protein;gremlin-1;hereditary mixed polyposis syndrome;HMPS;HMPS1;IHG-2;Increased in high glucose protein 2;increased in high glucose-2;MPSH;PIG2.
NCBI Gene Id26585
Protein NameGremlin-1
Description of TargetCytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner (By similarity). Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro) (PubMed:27036124). Acts as inhibitor of monocyte chemotaxis. Can inhibit the growth or viability of normal cells but not transformed cells when is overexpressed (By similarity).
Uniprot IDO60565
Protein Accession #NP_001178252
Nucleotide Accession #NM_001191323.1
Protein Size (# AA)184
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