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GRB2 Antibody (OAEB02404)

100 ug
$349.00
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Gene Symbol:
GRB2
NCBI Gene Id:
2885
Protein Name:
Growth factor receptor-bound protein 2
Swissprot Id:
P62993
Protein Accession #:
NP_002077.1
Alias Symbols:
GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP
Replacement Item:
This antibody may replace item sc-137056 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
WB, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GRB2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GRB2.
Immunogen:
This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Predicted Species Reactivity:
Cow, Dog, Human, Mouse, Pig, Rat, Zebrafish
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Zebrafish: 100%
Complete computational species homology data:
GRB2 Antibody (OAEB02404)
Peptide Sequence:
C-PRNYVTPVNRNV
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-GRB2 antibody - OAEB02404
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Approx 30kDa band seen in Jurkat lysate. Recommended for use at 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. Recommended concentration, 0.1-0.3ug/ml.
Target Reference:
* Lowenstein EJ, Daly RJ, Batzer AG, Li W, Margolis B, Lammers R, Ullrich A, Skolnik EY, Bar-Sagi D, Schlessinger J.; The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.; Cell. 1992 Aug 7;70(3):431-42.; Pubmed 1322798

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