GPX4 ELISA Kit (Rat) (OKCA00990)

Catalog No: OKCA00990

Size:96 Wells

Rat GPX4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  1:200	85	80-90
  1:400	102	97-107
  1:800	87	83-91
  1:1600	97	91-103

• ELISA Kit Principle: Aviva Systems Biology Gpx4 ELISA Kit (Rat) (OKCA00990) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Gpx4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gpx4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Gpx4 captured in well.
• ELISA Kit Range: 4.68 μU/mL-300 μU/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	83	80-86
  EDTA Plasma (n=4)	92	87-97

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Gpx4 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Gpx4 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • Gpx4 Microplate: 96 Wells (12 x 8 Well strips)
  • Gpx4 Lyophilized Standard: 2
  • 100X Biotinylated Gpx4 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 1.17 uU/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GPX4
• Gene Full Name: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
• Alias Symbols: Phgpx, gpx-4, snGpx, Gshpx-4
• NCBI Gene Id: 29328
• Protein Name: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
• Description of Target: Protects cells against membrane lipid peroxidation and cell death . Required for normal sperm development and male fertility . Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T cell response to viral and parasitic infection by protecting T cells from ferroptosis, a cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species, and by supporting T cell expansion.
• Uniprot ID: P36970
• Protein Accession #: NP_001034938.1
• Nucleotide Accession #: NM_001039849.2