Catalog No: OKDD00295 (Formerly GWB-KBBYP9)
Size:96 Wells
Price: $800.00
SKU
OKDD00295
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Datasheets/ManualsPrintable datasheet for GPX4 ELISA Kit (Human) (OKDD00295)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology GPX4 ELISA Kit (Human) (OKDD00295) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for GPX4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPX4 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GPX4 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GPX4 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GPX4 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-GPX4 Microplate96 Wells (12 x 8 Well Strips)
GPX4 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X GPX4 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell lysates, Cell culture aupernartes
Sensitivity< 0.061 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of GPX4. No significant cross-reactivity or interference between GPX4 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolGPX4
Gene Full Nameglutathione peroxidase 4
Alias Symbolsepididymis secretory sperm binding protein;Glutathione peroxidase 4;GPx-4;GSHPx-4;MCSP;PHGPx;phospholipid hydroperoxidase;phospholipid hydroperoxide glutathione peroxidase;phospholipid hydroperoxide glutathione peroxidase, mitochondrial;selenoprotein GPX4;SMDS;snGPx;snPHGPx;sperm nucleus glutathione peroxidase.
NCBI Gene Id2879
Protein NamePhospholipid hydroperoxide glutathione peroxidase
Description of TargetEssential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins (By similarity). Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide (By similarity). Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation (By similarity). Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species (PubMed:24439385). The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis (By similarity). The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures (By similarity). May be required to protect cells from the toxicity of ingested lipid hydroperoxides (By similarity). Required for normal sperm development and male fertility (By similarity). Essential for maturation and survival of photoreceptor cells (By similarity). Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion (By similarity).
Uniprot IDP36969
Protein Accession #NP_001034936.1
Nucleotide Accession #NM_001039847.2
Protein Size (# AA)197
Molecular Weight22 kDa
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