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Datasheets/Manuals | Printable datasheet for anti-GPX4 (ARP58473_P050) antibody |
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Tested Species Reactivity | Human, Rat | ||||||
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Goat, Guinea Pig, Yeast, Zebrafish | ||||||
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. | ||||||
Clonality | Polyclonal | ||||||
Host | Rabbit | ||||||
Application | IHC, WB | ||||||
Additional Information | IHC Information: Human heart (formalin-fixed, paraffin-embedded) stained with GPX4 antibody ARP58473_P050 at 5 ug/ml after heat-induced antigen retrieval | ||||||
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. | ||||||
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human GPX4 | ||||||
Purification | Affinity Purified | ||||||
Predicted Homology Based on Immunogen Sequence | Cow: 86%; Goat: 86%; Guinea Pig: 86%; Human: 100%; Mouse: 86%; Rat: 86%; Yeast: 93%; Zebrafish: 86% | ||||||
Peptide Sequence | Synthetic peptide located within the following region: QUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAA | ||||||
Concentration | 0.5 mg/ml | ||||||
Blocking Peptide | For anti-GPX4 (ARP58473_P050) antibody is Catalog # AAP58473 (Previous Catalog # AAPP34569) | ||||||
Sample Type Confirmation | GPX4 is supported by BioGPS gene expression data to be expressed in HepG2 | ||||||
Enhanced Validation |
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Reference | Peters,U., (2008) Cancer Epidemiol. Biomarkers Prev. 17 (5), 1144-1154 |
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Gene Symbol | GPX4 |
Gene Full Name | Glutathione peroxidase 4 |
Alias Symbols | MCSP, SMDS, GPx-4, PHGPx, snGPx, GSHPx-4, snPHGPx |
NCBI Gene Id | 2879 |
Protein Name | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial |
Description of Target | Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. |
Uniprot ID | P36969 |
Protein Accession # | NP_002076 |
Nucleotide Accession # | NM_002085 |
Protein Size (# AA) | 197 |
Molecular Weight | 19 kDa |
Protein Interactions | UBC; PRDX6; MAPK13; OTUD5; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GPX4 Antibody - middle region (ARP58473_P050)"?
The tested species reactivity for this item is "Human, Rat". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Goat, Guinea Pig, Yeast, Zebrafish".
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How long will it take to receive "GPX4 Antibody - middle region (ARP58473_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "GPX4 Antibody - middle region (ARP58473_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GPX4 Antibody - middle region (ARP58473_P050)"?
This target may also be called "MCSP, SMDS, GPx-4, PHGPx, snGPx, GSHPx-4, snPHGPx" in publications.
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What is the shipping cost for "GPX4 Antibody - middle region (ARP58473_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GPX4 Antibody - middle region (ARP58473_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GPX4 Antibody - middle region (ARP58473_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "19 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GPX4 Antibody - middle region (ARP58473_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GPX4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GPX4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GPX4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GPX4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GPX4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GPX4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.