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Catalog No: OKEH04801
Size:96T
Price: $625.00
SKU
OKEH04801
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology GPX3 ELISA Kit (Bovine) (OKEH04801) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-bovine GPX3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GPX3 and incubated. The GPX3 found in the sample or standards competes with the biotinylated GPX3 for limited binding sites on the immobilized anti-bovine GPX3 antibody. Excess unbound biotinylated GPX3 and sample or standard GPX3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GPX3 captured in the well and inversely proportional to the amount of GPX3 which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-GPX3 Microplate96 Wells (12 x 8 Well Strips)
GPX3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X GPX3-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.24 ng/mL
SpecificityNatural and recombinant Bovine Glutathione peroxidase 3
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolGPX3
Gene Full Nameglutathione peroxidase 3
Alias Symbolsglutathione peroxidase 3;glutathione peroxidase 3 (plasma);GPx-3;GPx-P;GSHPx-3;GSHPx-P;plasma glutathione peroxidase.
NCBI Gene Id281210
Protein NameP37141
Description of TargetGlutathione peroxidase 3
Uniprot IDhttps://www.uniprot.org/uniprot/P37141
Protein Accession #NP_776502.1
Nucleotide Accession #NM_174077.3
Protein Size (# AA)226
Molecular Weight27 kDa
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