GPX1 ELISA Kit (Pig) (OKCD02578)

Catalog No: OKCD02578

Size:96WELLS

Pig GPX1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-97%	91-101%	80-91%	87-103%
  EDTA Plasma (n=5)	79-98%	92-101%	96-104%	81-105%
  Heparin Plasma (n=5)	96-103%	94-101%	79-97%	81-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 1 (GPX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Peroxidase 1 (GPX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1 (GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione Peroxidase 1 (GPX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-98	83
  EDTA Plasma (n=5)	81-96	93
  Heparin Plasma(n=5)	91-105	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Peroxidase 1 (GPX1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Peroxidase 1 (GPX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GPX1 Microplate: 96 Wells (12 x 8 Well strips)
  • GPX1 Lyophilized Standard: 2
  • 100X Biotinylated GPX1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 2.81 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione Peroxidase 1 (GPX1). No significant cross-reactivity or interference between Glutathione Peroxidase 1 (GPX1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GPX1
• Gene Full Name: glutathione peroxidase 1
• Alias Symbols: cellular glutathione peroxidase;cytosolic glutathione peroxidase;glutathione peroxidase 1;GPx-1;GSHPx-1.
• NCBI Gene Id: 397403
• Protein Name: Glutathione peroxidase 1
• Description of Target: Protects the hemoglobin in erythrocytes from oxidative breakdown.
• Uniprot ID: Q8MJ14
• Protein Accession #: NP_999366.1
• Nucleotide Accession #: NM_214201.1
• Protein Size (# AA): 206