Catalog No: OKEH03059
Size:96T
Price: $600.00
SKU
OKEH03059
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GPT ELISA Kit (Mouse) (OKEH03059) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gpt has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Gpt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gpt captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.4% (n = 20)
Mean Inter-assay CV%: <= 8.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Gpt Microplate96 Wells (12 x 8 Well strips)
Gpt Lyophilized Standard2
100X Biotinylated Gpt Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Mouse Alanine aminotransferase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGpt
Gene Full Nameglutamic pyruvic transaminase, soluble
Alias Symbols1300007J06Rik;2310022B03Rik;A;alanine aminotransferase 1;ALT;ALT1;glutamate pyruvate transaminase 1;glutamic pyruvic transaminase 1, soluble;glutamic--alanine transaminase 1;glutamic--pyruvic transaminase 1;Gpt-;GPT 1;Gpt1;Gpt-1.
NCBI Gene Id76282
Protein NameQ8QZR5
Description of TargetAlanine aminotransferase 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q8QZR5
Protein Accession #NP_877957.1
Nucleotide Accession #NM_182805.3
Protein Size (# AA)496
Molecular Weight55 kDa
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