Catalog No: OKEH03059
Size:96T
Price: $600.00
SKU
OKEH03059
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Mouse, Mus musculus | ||||||||||||||||||||||
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Application | ELISA-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology GPT ELISA Kit (Mouse) (OKEH03059) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gpt has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Gpt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gpt captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <= 5.4% (n = 20) Mean Inter-assay CV%: <= 8.7% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.078 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Mouse Alanine aminotransferase 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Gpt |
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Gene Full Name | glutamic pyruvic transaminase, soluble |
Alias Symbols | 1300007J06Rik;2310022B03Rik;A;alanine aminotransferase 1;ALT;ALT1;glutamate pyruvate transaminase 1;glutamic pyruvic transaminase 1, soluble;glutamic--alanine transaminase 1;glutamic--pyruvic transaminase 1;Gpt-;GPT 1;Gpt1;Gpt-1. |
NCBI Gene Id | 76282 |
Protein Name | Q8QZR5 |
Description of Target | Alanine aminotransferase 1 |
Uniprot ID | https://www.uniprot.org/uniprot/Q8QZR5 |
Protein Accession # | NP_877957.1 |
Nucleotide Accession # | NM_182805.3 |
Protein Size (# AA) | 496 |
Molecular Weight | 55 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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