GPT ELISA Kit (Human) (OKCD06132)

Catalog No: OKCD06132

Size:96WELLS

GPT ELISA Kit (Human) (OKCD06132) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alanine Aminotransferase (ALT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alanine Aminotransferase (ALT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alanine Aminotransferase (ALT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alanine Aminotransferase (ALT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alanine Aminotransferase (ALT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alanine Aminotransferase (ALT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GPT Microplate: 96 Wells (12 x 8 Well strips)
  • GPT Lyophilized Standard: 2
  • 100X GPT HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.059ng/mL

Target Info

• Gene Symbol: GPT
• Gene Full Name: glutamic--pyruvic transaminase
• Alias Symbols: AAT1;alanine aminotransferase;alanine aminotransferase 1;alanine transaminase;ALT1;glutamate pyruvate transaminase 1;glutamic-alanine transaminase 1;Glutamic--alanine transaminase 1;glutamic-pyruvate transaminase (alanine aminotransferase);glutamic--pyruvic transaminase 1;GPT1.
• NCBI Gene Id: 2875
• Protein Name: Alanine aminotransferase 1
• Description of Target: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
• Uniprot ID: P24298
• Protein Accession #: NP_005300.1
• Nucleotide Accession #: NM_005309.2