GPT Chemi-Luminescent ELISA Kit (Rat) (OKCD03294)

Catalog No: OKCD03294

Size:96WELLS

Rat GPT ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	99-105%	85-99%	80-90%	86-104%
  EDTA Plasma (n=5)	92-101%	90-101%	94-102%	89-98%
  Heparin Plasma (n=5)	98-105%	80-101%	78-96%	96-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Alanine Aminotransferase (ALT). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Alanine Aminotransferase (ALT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alanine Aminotransferase (ALT) level in the sample or standard.
• ELISA Kit Range: 0.14-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95-104	101
  EDTA Plasma (n=5)	88-102	91
  Heparin Plasma (n=5)	95-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alanine Aminotransferase (ALT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alanine Aminotransferase (ALT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Gpt Microplate: 96 Wells (12 x 8 Well strips)
  • Gpt Lyophilized Standard: 2
  • 100X Gpt Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 1.23 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Gpt
• Gene Full Name: glutamic--pyruvic transaminase
• Alias Symbols: alanine aminotransferase 1;ALAT;ALT1;glutamate pyruvate transaminase 1;glutamic pyruvate transaminase;glutamic pyruvic transaminase (alanine aminotrasferase);glutamic pyruvic transaminase 1, soluble;glutamic--alanine transaminase 1;glutamic-pyruvate transaminase (alanine aminotransferase);glutamic--pyruvic transaminase 1;GPT 1;Gpt1.
• NCBI Gene Id: 81670
• Protein Name: Alanine aminotransferase 1
• Description of Target: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
• Uniprot ID: P25409
• Protein Accession #: NP_112301
• Nucleotide Accession #: NM_031039