GPSM3 Antibody (OAPB00813)

Catalog No: OAPB00813

Size:100UG

Western blot analysis of RNAse H2A in HeLa cell lysate with RNAse H2A antibody at 1 μg/mL.

Immunofluorescence of Rnse H2A in Hela cells with Rnse H2A antibody at 4.75 ug/mL.

Immunocytochemistry of RNAse H2A in HeLa cells with RNAse H2A antibody at 2 μg/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Liquid. RNAse H2A Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: RNAse H2A antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: RNAse H2A antibody was raised against a 17 amino acid synthetic peptide near the center of human RNAse H2A. The immunogen is located within amino acids 190 - 240 of RNAse H2A.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-GPSM3 (OAPB00813) antibody is Catalog # OEPB02936
• Application Info: RNAse H2A antibody can be used for detection of RNAse H2A by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Stein H and Hausen P. Enzyme from calf thymus degrading the RNA moiety of DNA-RNA hybrids: effect on DNA-dependent RNA polymerase. Science1969; 166:393-5.;Cerritelli SM and Crouch RJ. Ribonuclease H: the enzymes in eukaryotes. FEBS J.2009; 276:1494-505.;Jeong HS, Backlund PS, Chen HC, et al. RNase H2 of Saccharomyces cerevisiae is a complex of three proteins. Nuc. Acids Res.2004; 32:407-14. ;Crow YJ, Leitch A, Hayward BE, et al. Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutieres syndrome and mimic congenital viral brain infection. Nat. Genet.2006; 38:910-6.

Target Info

• Gene Symbol: RNASEH2A
• Gene Full Name: ribonuclease H2 subunit A
• Alias Symbols: AGS4;aicardi-Goutieres syndrome 4 protein;JUNB;ribonuclease H2 subunit A;ribonuclease H2, large subunit;ribonuclease HI large subunit;ribonuclease HI subunit A;RNase H(35);RNase H2 subunit A;RNase HI large subunit;RNASEHI;RNHIA;RNHL;THSD8.
• NCBI Gene Id: 10535
• Protein Name: Ribonuclease H2 subunit A
• Description of Target: Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.
• Uniprot ID: O75792
• Protein Accession #: NP_001263430.1; NP_071390.1
• Nucleotide Accession #: NM_001276501.1; NM_022107.1
• Molecular Weight: Predicted: 33 kDa Observed: 33 kDa