GPI1 ELISA Kit (Rat) (OKCD02516)

Catalog No: OKCD02516

Size:96WELLS

Rat GPI1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	83-103%	95-103%	79-90%	86-97%
  EDTA Plasma (n=5)	79-104%	80-90%	90-99%	94-105%
  Heparin Plasma (n=5)	98-105%	93-104%	85-93%	83-91%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Isomerase (GPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose 6 Phosphate Isomerase (GPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Isomerase (GPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glucose 6 Phosphate Isomerase (GPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-97	92
  EDTA Plasma (n=5)	84-98	93
  Heparin Plasma(n=5)	98-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose 6 Phosphate Isomerase (GPI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose 6 Phosphate Isomerase (GPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GPI1 Microplate: 96 Wells (12 x 8 Well strips)
  • GPI1 Lyophilized Standard: 2
  • 100X Biotinylated GPI1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 5.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glucose 6 Phosphate Isomerase (GPI). No significant cross-reactivity or interference between Glucose 6 Phosphate Isomerase (GPI) and analogues was observed
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Gpi
• Gene Full Name: glucose-6-phosphate isomerase
• Alias Symbols: Amf;autocrine motility factor;glucose-6-phosphate isomerase;Gpi1;Neuroleukin;Nlk;Pgi;Phi;Phosphoglucose isomerase;Phosphohexose isomerase.
• NCBI Gene Id: 292804
• Protein Name: Glucose-6-phosphate isomerase
• Description of Target: Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons (By similarity).
• Uniprot ID: Q6P6V0
• Protein Accession #: NP_032181.2
• Nucleotide Accession #: NM_008155.4
• Protein Size (# AA): 558