GPD2 Protein (OPPA01983)

Catalog No: OPPA01983 (Formerly GWB-E22300)

Size:2UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: GPD2 protein is supplied in 1x PBS and 50% glycerol. Physical appearance: Sterile Filtered clear solution.
• Host: E. Coli
  Product Introduction: GPD2 (Mitochondrial glycerol-3-phosphate dehydrogenase) is a Ca2+-sensitive, FAD-binding protein, which is located on the outer surface of the inner mitochondrial membrane. Two isoforms have been identified for mGPD: Isoform 1 is comprised of 727 a.a. residues, while isoform 2 lacks 126 a.a. residues of the N-terminus. GPD2 catalyses the oxidation of glycerol-3-phosphate to DHAP (dihydroxyacetone phosphate) with associated reduction of the enzyme-bound FAD. GPD2 is a testis-specific promoter of mitochondrial GPDH. GPD2 along with a cytosolic NAD-linked GPD forms the glycerol phosphate shuttle that uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, which results in the reoxidation of NADH produced during glycolysis.GPD2 deficiency contributes to the impairment of glucose-stimulated insulin discharge in a number of animal models of non-insulin dependent diabetes mellitus. GPD2 up-regulation as a result of a highly glycolytic environment contributes to the general increase in ROS generation and may lead to the progression of prostate cancer.
  Product Description: GPD2 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 558 amino acids fragment (43-600) corresponding to the GlpA domain fragment of the mature protein, having a total molecular mass of 64kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. The GPD2 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Please avoid freeze thaw cycles.
• Purity: Greater than 95.0% as determined by SDS-PAGE.

Target Info

• Gene Symbol: GPD2
• Alias Symbols: GDH2, GPDM, mGDH, mGPDH
• NCBI Gene Id: 2820
• Protein Name: Glycerol-3-phosphate dehydrogenase, mitochondrial
• Description of Target: Recombinant Human Glycerol-3-Phosphate Dehydrogenase 2
• Uniprot ID: P43304
• Protein Accession #: NP_000399.3
• Protein Size (# AA): Recombinant