GP5 ELISA Kit (Human) (OKCD07297)

Catalog No: OKCD07297

Size:96WELLS

GP5 ELISA Kit (Human) (OKCD07297) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster of Differentiation 42d (CD42d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster of Differentiation 42d (CD42d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster of Differentiation 42d (CD42d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster of Differentiation 42d (CD42d) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster of Differentiation 42d (CD42d) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster of Differentiation 42d (CD42d) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GP5 Microplate: 96 Wells (12 x 8 Well strips)
  • GP5 Lyophilized Standard: 2
  • 100X GP5 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma
• Sensitivity: < 0.122ng/mL

Target Info

• Gene Symbol: GP5
• Gene Full Name: glycoprotein V platelet
• Alias Symbols: CD42d;glycoprotein 5;GPV;platelet glycoprotein V.
• NCBI Gene Id: 2814
• Protein Name: Platelet glycoprotein V
• Description of Target: The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis.
• Uniprot ID: P40197
• Protein Accession #: NP_004479.1
• Nucleotide Accession #: NM_004488.2