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Catalog No: OCOA16691
Size:20UG
Price: $169.00
SKU
OCOA16691
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GP2 Protein Lysate (OCOA16691)

Datasheets/ManualsPrintable datasheet for GP2 Protein Lysate (OCOA16691)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionGP2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolGP2
Gene Full Nameglycoprotein 2
Alias Symbolsglycoprotein 2 (zymogen granule membrane);pancreatic secretory granule membrane major glycoprotein GP2;pancreatic zymogen granule membrane associated protein GP2;ZAP75.
NCBI Gene Id2813
Protein NameB7Z1G2, NM_001007242, NP_001007243
Description of TargetcDNA FLJ56017, highly similar to Pancreatic secretory granule membrane major glycoprotein GP2, Homo sapiens glycoprotein 2 (GP2), transcript variant 4, mRNA, pancreatic secretory granule membrane major glycoprotein GP2 isoform 4 precursor [Homo sapiens]
Uniprot IDB7Z1G2
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001007243
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001007242
Molecular Weight42.6 kDa
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