GP1BA ELISA Kit (Human) (OKCD07149)

Catalog No: OKCD07149

Size:96WELLS

GP1BA ELISA Kit (Human) (OKCD07149) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 42b (CD42b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 42b (CD42b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 42b (CD42b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 42b (CD42b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 42b (CD42b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 42b (CD42b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GP1BA Microplate: 96 Wells (12 x 8 Well strips)
  • GP1BA Lyophilized Standard: 2
  • 100X GP1BA HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma
• Sensitivity: < 0.35ng/mL

Target Info

• Gene Symbol: GP1BA
• Gene Full Name: glycoprotein Ib platelet subunit alpha
• Alias Symbols: antigen CD42b-alpha;BDPLT1;BDPLT3;BSS;CD42B;CD42b-alpha;DBPLT3;glycoprotein Ib (platelet), alpha polypeptide;glycoprotein Ib platelet alpha subunit;GP1B;GP-Ib alpha;GPIbA;GPIbalpha;mutant platelet membrane glycoprotein Ib-alpha;platelet glycoprotein Ib alpha chain;platelet membrane glycoprotein 1b-alpha subunit;platelet membrane glycoprotein Ib-alpha;VWDP.
• NCBI Gene Id: 2811
• Protein Name: Platelet glycoprotein Ib alpha chain
• Description of Target: GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.
• Uniprot ID: P07359
• Protein Accession #: NP_000164.5
• Nucleotide Accession #: NM_000173.6