GOT1 ELISA Kit (Rat) (OKCA00950)

Catalog No: OKCA00950

Size:96 Wells

Rat GOT1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	90	87-92
  1:2	104	100-107
  1:4	93	88-97
  1:8	98	92-103

• ELISA Kit Principle: Aviva Systems Biology GOT1 ELISA Kit (Rat) (OKCA00950) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for GOT1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GOT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GOT1 captured in well.
• ELISA Kit Range: 3.12 mIU/mL-200 mIU/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94	90-98
  EDTA Plasma (n=4)	95	91-98

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level GOT1 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level GOT1 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • GOT1 Microplate: 96 Wells (12 x 8 Well strips)
  • GOT1 Lyophilized Standard: 2
  • 100X Biotinylated GOT1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma
• Sensitivity: 0.78 mIU/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GOT1
• Gene Full Name: Aspartate aminotransferase, cytoplasmic
• Protein Name: Aspartate aminotransferase, cytoplasmic
• Description of Target: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
• Uniprot ID: P12343
• Protein Accession #: XP_002718676.1
• Nucleotide Accession #: XM_002718630.3