Catalog No: OKEH06988
Size:96T
Price: $525.00
SKU
OKEH06988
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GOT1 ELISA Kit (Human) (OKEH06988)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology GOT1 ELISA Kit (Human) (OKEH06988) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GOT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GOT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GOT1 captured in the well.
ELISA Kit Range1.56-100U/L
ELISA Kit RecoveryMean recovery when spiking into matrix = 95%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <4.9%
Inter-Assay CV <6.2%
ELISA Kit Component
ComponentAmount
GOT1 Microplate96 Wells (12 x 8 Well strips)
GOT1 Lyophilized Standard2 x 100 U
100X GOT1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.87U/L
SpecificityNatural and recombinant Human Aspartate aminotransferase, cytoplasmic
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGOT1
Gene Full Nameglutamic-oxaloacetic transaminase 1
Alias Symbolsaspartate aminotransferase 1;aspartate aminotransferase, cytoplasmic;aspartate transaminase 1;AST1;ASTQTL1;cAspAT;cCAT;cysteine aminotransferase, cytoplasmic;cysteine transaminase, cytoplasmic;GIG18;glutamate oxaloacetate transaminase 1;glutamic-oxaloacetic transaminase 1, soluble;growth-inhibiting protein 18;testis secretory sperm-binding protein Li 196a;transaminase A.
NCBI Gene Id2805
Protein NameP17174
Description of TargetAspartate aminotransferase, cytoplasmic
Uniprot IDhttps://www.uniprot.org/uniprot/P17174
Protein Accession #NP_002070
Nucleotide Accession #NM_002079
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