Catalog No: OKCD02116 (Formerly GWB-KBBUM1)
Size:96WELLS
Price: $750.00
SKU
OKCD02116
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Datasheets/ManualsPrintable datasheet for GOLGA8A ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Golgin A8 Family, Member A (GOLGA8A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Golgin A8 Family, Member A (GOLGA8A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Golgin A8 Family, Member A (GOLGA8A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Golgin A8 Family, Member A (GOLGA8A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Golgin A8 Family, Member A (GOLGA8A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Golgin A8 Family, Member A (GOLGA8A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GOLGA8A Microplate96 Wells (12 x 8 Well Strips)
GOLGA8A Lyophilized Standard2 x 20 ng
100X Biotinylated GOLGA8A Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.116 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Golgin A8 Family, Member A (GOLGA8A). No significant cross-reactivity or interference between Golgin A8 Family, Member A (GOLGA8A) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGOLGA8A
Gene Full NameGolgin A8 family member A
Alias SymbolsGM88, FAP286, CFAP286, GOLGA8B
NCBI Gene Id23015
Protein NameGolgin subfamily A member 8A
Description of TargetMay be involved in maintaining Golgi structure.
Uniprot IDA7E2F4
Protein Accession #NP_851422.1
Nucleotide Accession #NM_181077.3
Protein Size (# AA)631
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