Donkey Anti-Goat IgG [H&L] Polyclonal Antibody - TRITC Conjugated (OARA03953)

Catalog No: OARA03953

Size:500UL

Product Info

• Predicted Species Reactivity: Goat
• Product Format: Lyophilized
• Clonality: Polyclonal
• Host: Donkey
• Conjugation: TRITC
• Application: Dot blot|Flow cytometry|Fluorescence-linked immunosorbent assay|Immunofluorescence
  Excitation & Emission Wavelength: 550-570nm
  F/P Ratio: 2.1 moles Rhodamine (TRITC) per mole of IgG F(ab')2
  Description: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Reconstitution and Storage: 2°C to 8°C|-20°C or -80°C
• Immunogen: Goat IgG whole molecule
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
• Concentration: 1 mg/ml
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
• Application Info: FLISA: 1:10000~50000
  IF: 1:1000~5000

Target Info

• Description of Target: F(ab')2 Anti-Goat IgG Rhodamine Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
• Molecular Weight: 444 kDa