Goat Anti-Mouse Kappa Antibody (OASB01553)

Catalog No: OASB01553

Size:500UG

Product Info

• Tested Species Reactivity: Mouse
• Predicted Species Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Host: Goat
• Conjugation: LE: Low Endotoxin, AF: Azide Free
• Application: FC, IHC-P, ELISPOT, STIM
• Additional Information: Description: Pepsin digest of Goat Anti-Mouse Kappa - Pooled antisera from goats hyperimmunized with mouse kappa light chains; purified by affinity chromatography on mouse kappa light chains covalently linked to agarose; reacts with mouse kappa light chains; cross-adsorbed against mouse lambda light chains for minimal reactivity
• Reconstitution and Storage: Store at -20C
• Immunogen: Mouse kappa light chains
• Purification: Gel filtration chromatography of pepsin digested antibody
• Concentration: 0.5 mg/mL
• Specificity: Kappa
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance with characteristics of a standard reference reagent.
• Warning: Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection whenhandling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discarding to avoid accumulation ofpotentially explosive deposits in lead or copper plumbing.
• Dilution: ELISA: Purified antibody
  HRP conjugate
  1 ug
  1:4,000-1:8,002
• Application Info: Enzyme-Linked-Immunosorbent-Assay (ELISA), Immunoblotting, Immunohistochemistry
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
  - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C.
• Cross Absorption: Pooled mouse paraproteins with λ light chains
  Purification: Gel filtration chromatography of pepsin digested

Target Info

• Gene Symbol: IGK
• Gene Full Name: Ig kappa chain C region
• Alias Symbols: k, kappa
• NCBI Gene Id: 243469
• Description of Target: Summary:Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the kappa light chain locus from the C57BL/6J inbred mouse strain. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments in this cluster are incapable of encoding a protein and are considered pseudogenes.
• Uniprot ID: P01837