Goat Anti-Mouse IgG2a Antibody - Biotin Conjugated (OASB01602)

Catalog No: OASB01602

Size:500UG

ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Mouse IgG2a, Human ads-BIOT followed by Streptavidin-HRP.

Product Info

• Tested Species Reactivity: Mouse
• Predicted Species Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Host: Goat
• Conjugation: Biotin
• Application: FC, IHC-F
• Additional Information: Description: Pepsin digest of Goat Anti-Mouse IgG2a, Human ads - Pooled antisera from goats hyperimmunized with mouse IgG2a; purified by affinity chromatography on mouse IgG2a covalently linked to agarose; reacts with the heavy chain of mouse IgG2a; cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA / human immunoglobulins and pooled sera for minimal reactivity
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Mouse IgG2a
• Purification: Gel filtration chromatography of pepsin digested antibody.
• Concentration: 0.5 mg/mL
• Specificity: IgG2a
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance with characteristics of a standard reference reagent.
• Warning: Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Immunofluorescence:
  ELISA:
  FITC conjugate
  BIOT conjugate
  R-PE conjugate
  BIOT conjugate
  <= 1 ug/10⁶ cells
  <= 1 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
  1:5,000-1:10,000
• Application Info: Indirect immunofluorescent staining in conjunction with mouse IgG2a monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
  - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
  - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
• Cross Absorption: Mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified humanparaproteins.

Target Info

• Gene Symbol: IGHG2A
• Gene Full Name: Ig gamma-2 chain C region
• Alias Symbols: Igh-, Igh-1, Igh-1a, 1810060O09Rik
• NCBI Gene Id: 380793
• Uniprot ID: P01863, P01864, P01865