Mouse IgG (H+L), Human ads Antibody : PE/CY5.5 (OASB01522)

Catalog No: OASB01522

Size:250ug

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat F(ab’)2 Anti-Mouse IgG(H+L), Human ads-PE/CY5.5.

Product Info

• Tested Species Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Host: Goat
• Conjugation: PE: Phycoerythrin, CY5.5: Cyanine 5.5
• Application: FC
• Additional Information: Description: Pepsin digest of Goat Anti-Mouse IgG(H+L), Human ads - Pooled antisera from goats hyperimmunized with mouse IgG; purified by affinity chromatography on mouse IgG covalently linked to agarose; reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA; cross-adsorbed against human immunoglobulins and pooled sera for minimal reactivity
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Mouse IgG
• Purification: Gel filtration chromatography of pepsin digested antibody.
• Concentration: 0.25 mg/mL
• Specificity: IgG(H+L)
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance with characteristics of a standard reference reagent.
• Warning: - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C.- The fluorescein (FITC) and Cyanine 5 (CY5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3.Store at 2- 8 C.- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20C.- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20C.- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C.- The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!- The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 5.5 (R- PE- CY5.5) conjugates are suppliedas 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
• Dilution: Immunofluorescence:
  ELISA:
  FITC conjugate
  BIOT conjugate
  R-PE, APC, SPRD, CY5 and
  R-PE-CY5.5 conjugates
  AP conjugate
  HRP conjugate
  BIOT conjugate
  <= 1 ug/10⁶ cells
  <= 1 ug/10⁶ cells
  <= 0.1 ug/10⁶ cells
  1:2,000-1:4,000
  1:4,000-1:8,000
  1:5,000-1:20,000
• Application Info: Indirect immunofluorescent staining in conjunction with mouse monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3
  Store at 2- 8 C
  - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
  - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
  - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5) conjugates are suppliedas 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
• Cross Absorption: Pooled human sera and purified human paraproteins.

Target Info

• Gene Full Name: Ig gamma-1 chain C region